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| Sorgatto,Vanessa Ghiggi; Carvalho,Julio Cesar de; Sydney,Eduardo Bittencourt; Medeiros,Adriane Bianchi Pedroni; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo. |
| Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial oil; Ethanolysis; FAEE; Microscale; Screening. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401 |
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| Herlory, Olivier; Guarini, Jean-marc; Richard, Pierre; Blanchard, Gerard. |
| The spatio-temporal dynamics of the microphytobenthic biofilm was analysed at microscale over a complete day-time emersion period in an intertidal mudflat. The structures of the biofilm were monitored by analysing the variability of the epipelic diatom cover in the horizontal dimension and the kinetics of biomass dispersion in the vertical dimension. Colonisation of the sediment surface by diatoms was rapid (within 15 min); 75% of the biomass contained in the top 1 mm was concentrated in the upper 200 mum, but the cover never reached 100%. This biomass had a higher chlorophyll a/pheopigment ratio than deeper in the sediment, suggesting a better physiological state and a higher photosynthetic potential. The dynamics of the biomass in the upper 200 mum... |
| Tipo: Text |
Palavras-chave: Microphytobenthos; Biofilm; Intertidal mudflat; Spatio temporal dynamics; Microscale. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00000/10905/7580.pdf |
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| Basterretxea, G.; Font-munoz, Joan Salvador; Tuval, I. |
| Phytoplankton are by definition autotrophic microorganisms that passively drift with fluid motion. Accordingly, the traditional view of a turbulence-homogenized phytoplankton distribution in the ocean, where cells randomly organize and interact, is deeply rooted in biological oceanography studies. However, increasing understanding of microscopic processes in the ocean is revealing a world of microscale patterns resulting from cell behaviors and fluid-cell interactions that challenges this vision. Autotrophic cells have developed active (i.e., flagella) and passive (i.e., morphological structures and vesicles) motility mechanisms that allow them different degrees of spatial control. Their complex interaction with the ocean physicochemical landscape commonly... |
| Tipo: Text |
Palavras-chave: Phytoplankton; Microscale; Turbulence; Orientation; Shear. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00621/73261/72476.pdf |
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